The aim of this study was to investigate the protective effects of selenium yeast (Se-Y) against hepatotoxicity induced by ochratoxin A (OTA).The OTA-induced liver injury model was established in chickens by daily oral gavage of 50 µg/kg OTA for 21 days.Serum biochemistry analysis, antioxidant analysis, as well as the qRT-PCR and Western blot (WB) analyses were Consumers behaviour of students when shopping for organic food in the Czech Republic then used to evaluate oxidative damage and apoptosis in chicken liver tissue.
The results showed that Se-Y significantly increased liver coefficient induced by OTA (P < 0.05).OTA + Se-Y treated group revealed that Se-Y reduced the OTA-induced increase in glutamic pyruvic transaminase (ALT), glutamic oxaloacetic transaminase (AST) and malonaldehyde (MDA) content, and reversed the decrease in antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) (P < 0.
05).In this study, we found that OTA is involved in the mRNA expression levels about Nrf2/Keap1 and PI3K/AKT signaling pathways, such as oxidative stress-related genes (Nrf2, GSH-Px, GLRX2 and Keap1) and apoptosis-related genes (Bax, Caspase3, P53, AKT, PI3K and Bcl-2).Besides, significant downregulations of protein expression of HO-1, MnSOD, Nrf2 and Bcl-2, Comparative analysis on forecasting methods and how to choose a suitable one: case study in financial time series as well as a significant upregulation of Caspase3 and Bax levels were observed after contaminated with OTA (P < 0.
05).Notably, OTA-induced apoptosis and oxidative damage in the liver of chickens were reverted back to normal level in the OTA + Se-Y group.Our findings indicate that pretreatment with Se-Y effectively ameliorates OTA-induced hepatotoxicity.